Raymond James Stadium - February 8, 2025
| Position | # | Rider | Interval | Best Time |
|---|---|---|---|---|
| 1 | 4 | Chase Sexton | 54.17 | |
| 2 | 2 | Cooper Webb | 0.364 | 54.54 |
| 3 | 27 | Malcolm Stewart | 0.571 | 54.75 |
| 4 | 96 | Hunter Lawrence | 0.594 | 54.77 |
| 5 | 32 | Justin Cooper | 0.716 | 54.89 |
| 6 | 21 | Jason Anderson | 0.899 | 55.07 |
| 7 | 17 | Joey Savatgy | 1.055 | 55.23 |
| 8 | 94 | Ken Roczen | 1.252 | 55.43 |
| 9 | 51 | Justin Barcia | 1.375 | 55.55 |
| 10 | 57 | Benny Bloss | 1.469 | 55.64 |
| 11 | 46 | Justin Hill | 1.536 | 55.71 |
| 12 | 3 | Eli Tomac | 1.558 | 55.73 |
| 13 | 49 | Mitchell Oldenburg | 1.585 | 55.76 |
| 14 | 7 | Aaron Plessinger | 1.668 | 55.84 |
| 15 | 77 | Jerry Robin | 2.750 | 56.92 |
| 16 | 12 | Shane McElrath | 3.142 | 57.32 |
| 17 | 11 | Kyle Chisholm | 3.397 | 57.57 |
| 18 | 146 | Kevin Moranz | 3.656 | 57.83 |
| 19 | 45 | Colt Nichols | 3.832 | 58.01 |
| 20 | 86 | Mitchell Harrison | 4.801 | 58.98 |