Raymond James Stadium - February 8, 2025
| Position | # | Rider | Interval | Best Time |
|---|---|---|---|---|
| 1 | 3 | Eli Tomac | 55.26 | |
| 2 | 2 | Cooper Webb | 0.253 | 55.51 |
| 3 | 27 | Malcolm Stewart | 0.832 | 56.09 |
| 4 | 4 | Chase Sexton | 1.079 | 56.34 |
| 5 | 21 | Jason Anderson | 1.201 | 56.46 |
| 6 | 17 | Joey Savatgy | 1.327 | 56.59 |
| 7 | 7 | Aaron Plessinger | 1.467 | 56.73 |
| 8 | 51 | Justin Barcia | 1.805 | 57.07 |
| 9 | 94 | Ken Roczen | 1.809 | 57.07 |
| 10 | 96 | Hunter Lawrence | 1.988 | 57.25 |
| 11 | 32 | Justin Cooper | 2.039 | 57.30 |
| 12 | 46 | Justin Hill | 2.210 | 57.47 |
| 13 | 49 | Mitchell Oldenburg | 2.299 | 57.56 |
| 14 | 57 | Benny Bloss | 3.125 | 58.39 |
| 15 | 77 | Jerry Robin | 3.439 | 58.70 |
| 16 | 146 | Kevin Moranz | 4.334 | 59.59 |
| 17 | 78 | Cade Clason | 4.542 | 59.80 |
| 18 | 12 | Shane McElrath | 4.583 | 59.84 |
| 19 | 11 | Kyle Chisholm | 4.605 | 59.87 |
| 20 | 86 | Mitchell Harrison | 5.526 | 1:00.79 |
| 21 | 45 | Colt Nichols | 6.001 | 1:01.26 |