Acrisure Stadium - April 26, 2025
| Position | # | Rider | Interval | Best Time |
|---|---|---|---|---|
| 1 | 4 | Chase Sexton | 47.27 | |
| 2 | 2 | Cooper Webb | 0.434 | 47.71 |
| 3 | 27 | Malcolm Stewart | 0.450 | 47.72 |
| 4 | 94 | Ken Roczen | 0.650 | 47.92 |
| 5 | 32 | Justin Cooper | 0.796 | 48.07 |
| 6 | 46 | Justin Hill | 0.835 | 48.11 |
| 7 | 7 | Aaron Plessinger | 1.583 | 48.85 |
| 8 | 14 | Dylan Ferrandis | 1.698 | 48.97 |
| 9 | 17 | Joey Savatgy | 1.833 | 49.10 |
| 10 | 15 | Dean Wilson | 1.860 | 49.13 |
| 11 | 12 | Shane McElrath | 2.400 | 49.67 |
| 12 | 28 | Christian Craig | 2.407 | 49.68 |
| 13 | 45 | Colt Nichols | 2.950 | 50.22 |
| 14 | 72 | Anthony Rodriguez | 3.082 | 50.35 |
| 15 | 49 | Mitchell Oldenburg | 3.251 | 50.52 |
| 16 | 57 | Benny Bloss | 3.495 | 50.77 |
| 17 | 33 | Fredrik Noren | 3.608 | 50.88 |
| 18 | 43 | Grant Harlan | 3.764 | 51.03 |
| 19 | 122 | Justin Starling | 3.781 | 51.05 |
| 20 | 86 | Mitchell Harrison | 3.800 | 51.07 |
| 21 | 11 | Kyle Chisholm | 3.873 | 51.14 |
| 22 | 146 | Kevin Moranz | 4.124 | 51.40 |