Lucas Oil Stadium - March 8, 2025
| Position | # | Rider | Interval | Best Time |
|---|---|---|---|---|
| 1 | 94 | Ken Roczen | 48.72 | |
| 2 | 4 | Chase Sexton | 0.057 | 48.78 |
| 3 | 2 | Cooper Webb | 0.193 | 48.92 |
| 4 | 21 | Jason Anderson | 0.297 | 49.02 |
| 5 | 27 | Malcolm Stewart | 0.367 | 49.09 |
| 6 | 32 | Justin Cooper | 0.654 | 49.38 |
| 7 | 14 | Dylan Ferrandis | 0.812 | 49.53 |
| 8 | 46 | Justin Hill | 1.008 | 49.73 |
| 9 | 7 | Aaron Plessinger | 1.508 | 50.23 |
| 10 | 51 | Justin Barcia | 1.984 | 50.71 |
| 11 | 57 | Benny Bloss | 2.061 | 50.78 |
| 12 | 17 | Joey Savatgy | 2.473 | 51.20 |
| 13 | 45 | Colt Nichols | 2.556 | 51.28 |
| 14 | 49 | Mitchell Oldenburg | 2.709 | 51.43 |
| 15 | 12 | Shane McElrath | 2.751 | 51.47 |
| 16 | 72 | Anthony Rodriguez | 2.916 | 51.64 |
| 17 | 77 | Jerry Robin | 3.536 | 52.26 |
| 18 | 146 | Kevin Moranz | 4.317 | 53.04 |
| 19 | 11 | Kyle Chisholm | 4.613 | 53.34 |
| 20 | 122 | Justin Starling | 4.673 | 53.40 |
| 21 | 86 | Mitchell Harrison | 4.883 | 53.61 |
| 22 | 33 | Fredrik Noren | 5.021 | 53.74 |