AT&T Stadium - February 22, 2025
| Position | # | Rider | Interval | Best Time |
|---|---|---|---|---|
| 1 | 27 | Malcolm Stewart | 49.11 | |
| 2 | 4 | Chase Sexton | 0.196 | 49.30 |
| 3 | 32 | Justin Cooper | 0.553 | 49.66 |
| 4 | 2 | Cooper Webb | 0.640 | 49.74 |
| 5 | 7 | Aaron Plessinger | 0.660 | 49.77 |
| 6 | 21 | Jason Anderson | 0.860 | 49.97 |
| 7 | 94 | Ken Roczen | 0.946 | 50.05 |
| 8 | 17 | Joey Savatgy | 1.155 | 50.26 |
| 9 | 51 | Justin Barcia | 1.232 | 50.34 |
| 10 | 14 | Dylan Ferrandis | 1.375 | 50.48 |
| 11 | 57 | Benny Bloss | 1.518 | 50.62 |
| 12 | 49 | Mitchell Oldenburg | 1.575 | 50.68 |
| 13 | 46 | Justin Hill | 2.270 | 51.37 |
| 14 | 45 | Colt Nichols | 2.497 | 51.60 |
| 15 | 77 | Jerry Robin | 2.517 | 51.62 |
| 16 | 12 | Shane McElrath | 2.600 | 51.71 |
| 17 | 72 | Anthony Rodriguez | 2.884 | 51.99 |
| 18 | 122 | Justin Starling | 4.093 | 53.20 |
| 19 | 86 | Mitchell Harrison | 4.603 | 53.71 |
| 20 | 33 | Fredrik Noren | 4.979 | 54.08 |
| 21 | 146 | Kevin Moranz | 5.730 | 54.84 |
| 22 | 11 | Kyle Chisholm | 6.410 | 55.52 |